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phusiontm high fidelity dna polymerase  (New England Biolabs)


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    Structured Review

    New England Biolabs phusiontm high fidelity dna polymerase
    Phusiontm High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phusion+high+fidelity+polymerase/Phusion+High-Fidelity+DNA+Polymerase/pmc13010419-8-0-5
    Average 99 stars, based on 16570 article reviews
    phusiontm high fidelity dna polymerase - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Presurgical levels of circulating tumour DNA in patients with resectable chemotherapy-naïve colorectal liver metastases: association with multiorgan recurrence and survival in the MIRACLE cohort.
    Article Snippet: Background: Almost half of patients with colorectal liver metastases (CRLM) experience disease recurrence within one year after treatment with curative intent.. To improve treatment, upfront identification of patients with CRLM at high risk of rapid disease recurrence is crucial.. Methods: In this retrospective cohort-study, pretreatment ctDNA levels were determined by the modified fast aneuploidy screening test-sequencing system (mFast-SeqS) in 182 patients with resectable CRLM who did not receive perioperative chemotherapy.

    Article Title: The human DEAD-box protein DDX3X regulates host and viral mRNA translation during Sendai Virus infection
    Article Snippet: .. 5’UTR regions of interest were amplified from either gDNA or cDNA using Phusion high-fidelity polymerase (NEB, M0530S) according to the manufacturer’s instructions, using PCR primers with 15 nt homologous regions to the NcoI and HindIII restriction sites of the pGL3-Prom vector (Promega, E1761). ..

    Article Title: Functional characterization of bat limb regulatory elements
    Article Snippet: Total RNA samples were enriched for poly-A containing transcripts using the Oligotex mRNA Mini kit (Qiagen) and strand-specific RNA-seq libraries were generated using PrepX RNA library preparation kits (IntegenX) following the manufacturer’s protocol. .. After clean up with AMPure XP beads (Beckman Coulter) and amplification with Phusion High-Fidelity polymerase (NEB), RNA libraries were sequenced on a HiSeq 2500 to a depth of at least 30 M reads. ..

    Article Title: Presurgical levels of circulating tumour DNA in patients with resectable chemotherapy-naïve colorectal liver metastases: association with multiorgan recurrence and survival in the MIRACLE cohort
    Article Snippet: .. In short, 1 ng of cfDNA was amplified using a Phusion high-fidelity polymerase (New England Biolabs) by a single primary primer pair for specific amplification of LINE-1 sequences throughout the genome (2′ at 98 °C, followed by 8 cycles of 10′ at 98 °C, 2′ at 57 °C and 2′ at 72 °C). ..

    Plasmid Preparation:

    Article Title: Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus
    Article Snippet: Triple MAB_2736c or NT plasmids were transformed into ATCC19977 and selected on 7H10 + 0.5% (vol/vol) glycerol + 10% (vol/vol) OADC agar plates containing 50 μg mL −1 kanamycin sulfate. .. MAB_2736c constitutive plasmid was constructed into pL5 PTetO Msm PonA1 truncation A-FLAG clone 1 ( ) using NdeI (R0111, NEB) and HindIII-HF (R3104, NEB) restriction digest, followed by isothermal assembly with Phusion High-Fidelity Polymerase (M0530, NEB) and transformed into ATCC19977 and M. smegmatis . ..

    Article Title: The human DEAD-box protein DDX3X regulates host and viral mRNA translation during Sendai Virus infection
    Article Snippet: .. 5’UTR regions of interest were amplified from either gDNA or cDNA using Phusion high-fidelity polymerase (NEB, M0530S) according to the manufacturer’s instructions, using PCR primers with 15 nt homologous regions to the NcoI and HindIII restriction sites of the pGL3-Prom vector (Promega, E1761). ..

    Construct:

    Article Title: Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus
    Article Snippet: Triple MAB_2736c or NT plasmids were transformed into ATCC19977 and selected on 7H10 + 0.5% (vol/vol) glycerol + 10% (vol/vol) OADC agar plates containing 50 μg mL −1 kanamycin sulfate. .. MAB_2736c constitutive plasmid was constructed into pL5 PTetO Msm PonA1 truncation A-FLAG clone 1 ( ) using NdeI (R0111, NEB) and HindIII-HF (R3104, NEB) restriction digest, followed by isothermal assembly with Phusion High-Fidelity Polymerase (M0530, NEB) and transformed into ATCC19977 and M. smegmatis . ..

    Restriction Assay:

    Article Title: Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus
    Article Snippet: Triple MAB_2736c or NT plasmids were transformed into ATCC19977 and selected on 7H10 + 0.5% (vol/vol) glycerol + 10% (vol/vol) OADC agar plates containing 50 μg mL −1 kanamycin sulfate. .. MAB_2736c constitutive plasmid was constructed into pL5 PTetO Msm PonA1 truncation A-FLAG clone 1 ( ) using NdeI (R0111, NEB) and HindIII-HF (R3104, NEB) restriction digest, followed by isothermal assembly with Phusion High-Fidelity Polymerase (M0530, NEB) and transformed into ATCC19977 and M. smegmatis . ..

    Transformation Assay:

    Article Title: Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus
    Article Snippet: Triple MAB_2736c or NT plasmids were transformed into ATCC19977 and selected on 7H10 + 0.5% (vol/vol) glycerol + 10% (vol/vol) OADC agar plates containing 50 μg mL −1 kanamycin sulfate. .. MAB_2736c constitutive plasmid was constructed into pL5 PTetO Msm PonA1 truncation A-FLAG clone 1 ( ) using NdeI (R0111, NEB) and HindIII-HF (R3104, NEB) restriction digest, followed by isothermal assembly with Phusion High-Fidelity Polymerase (M0530, NEB) and transformed into ATCC19977 and M. smegmatis . ..

    Polymerase Chain Reaction:

    Article Title: The human DEAD-box protein DDX3X regulates host and viral mRNA translation during Sendai Virus infection
    Article Snippet: .. 5’UTR regions of interest were amplified from either gDNA or cDNA using Phusion high-fidelity polymerase (NEB, M0530S) according to the manufacturer’s instructions, using PCR primers with 15 nt homologous regions to the NcoI and HindIII restriction sites of the pGL3-Prom vector (Promega, E1761). ..

    Article Title: RNA localization to nuclear speckles follows splicing logic
    Article Snippet: For reverse transcription, 200 ng of RNA was used for a 10 μl reaction using SuperScript IV (Thermo Fisher, 18090010) with gene-specific primers at 100 nM concentration for 1 h, according to the manufacturer’s protocol. .. PCR reactions were carried out in an Eppendorf X50S 96-well thermocycler using Phusion High Fidelity polymerase (New England Biolabs, M0530L). ..



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